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Chem Impex International
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Inserm Transfert
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Miltenyi Biotec
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Image Search Results
Journal: PLOS ONE
Article Title: Robust isolation protocol for mouse leukocytes from blood and liver resident cells for immunology research
doi: 10.1371/journal.pone.0304063
Figure Lengend Snippet: After sample collection, both blood and liver are processed. Left lateral liver lobe is cut and digested on a GentleMACS Dissociator followed by centrifugation separation of hepatocytes and intra-hepatic cells. Lymphocytes are purified using a Percoll gradient to remove debris and remaining hepatocytes. Hepatocytes are pelleted for debris removal. After cell counting, cells are ready for all downstream applications. The blood samples are collected from the saphenous vein, after a filtering step, blood is incubated with magnetic beads for red blood cell depletion. After washing, cells are counted and ready for downstream applications. Image created with BioRender.
Article Snippet: Briefly, the left lateral lobe from mouse liver was carefully dissected and inserted in the
Techniques: Centrifugation, Purification, Cell Counting, Incubation, Magnetic Beads